Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV) : Identification of neutralizing and antibodies reactive to S, N, M and E viral proteins
Identifieur interne : 004D88 ( Main/Exploration ); précédent : 004D87; suivant : 004D89Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV) : Identification of neutralizing and antibodies reactive to S, N, M and E viral proteins
Auteurs : Ralph A. Tripp [États-Unis] ; Lia M. Haynes [États-Unis] ; Deborah Moore [États-Unis] ; Barbara Anderson [États-Unis] ; Azaibi Tamin [États-Unis] ; Brian H. Harcourt [États-Unis] ; Les P. Jones [États-Unis] ; Mamadi Yilla [États-Unis] ; Gregory J. Babcock [États-Unis] ; Thomas Greenough [États-Unis] ; Donna M. Ambrosino [États-Unis] ; Rene Alvarez [États-Unis] ; Justin Callaway [États-Unis] ; Sheana Cavitt [États-Unis] ; Kurt Kamrud [États-Unis] ; Harold Alterson [États-Unis] ; Jonathan Smith [États-Unis] ; Jennifer L. Harcoun [États-Unis] ; CONGRONG MIAO [États-Unis] ; Raj Razdan [États-Unis] ; James A. Comer [États-Unis] ; Pierre E. Rollin [États-Unis] ; Thomas G. Ksiazek [États-Unis] ; Anthony Sanchez [États-Unis] ; Paul A. Rota [États-Unis] ; William J. Bellini [États-Unis] ; Larry J. Anderson [États-Unis]Source :
- Journal of virological methods [ 0166-0934 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (biosynthèse), Anticorps antiviraux (immunologie), Anticorps monoclonaux (biosynthèse), Anticorps monoclonaux (immunologie), Cartographie épitopique, Cellules Vero, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (immunologie), Humains, Lignée cellulaire, Protéines de l'enveloppe virale (immunologie), Protéines de la matrice virale (immunologie), Protéines nucléocapside (immunologie), Protéines virales structurales (immunologie), Spécificité des anticorps, Syndrome respiratoire aigu sévère (virologie), Tests de neutralisation, Virus du SRAS (immunologie).
- MESH :
- biosynthèse : Anticorps antiviraux, Anticorps monoclonaux.
- immunologie : Anticorps antiviraux, Anticorps monoclonaux, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines de la matrice virale, Protéines nucléocapside, Protéines virales structurales, Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- Pascal (Inist)
- Animaux, Cartographie épitopique, Cellules Vero, Glycoprotéine de spicule des coronavirus, Humains, Lignée cellulaire, Spécificité des anticorps, Tests de neutralisation, Virus syndrome respiratoire aigu sévère, Anticorps monoclonal, Identification, Anticorps neutralisant, Protéine, Déterminant antigénique, Microbiologie, Méthode, Virologie.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (biosynthesis), Antibodies, Monoclonal (immunology), Antibodies, Viral (biosynthesis), Antibodies, Viral (immunology), Antibody Specificity, Antigenic determinant, Cell Line, Chlorocebus aethiops, Epitope Mapping, Humans, Identification, Membrane Glycoproteins (immunology), Method, Microbiology, Monoclonal antibody, Neutralization Tests, Neutralizing antibody, Nucleocapsid Proteins (immunology), Protein, SARS Virus (immunology), Severe Acute Respiratory Syndrome (virology), Severe acute respiratory syndrome virus, Spike Glycoprotein, Coronavirus, Vero Cells, Viral Envelope Proteins (immunology), Viral Matrix Proteins (immunology), Viral Structural Proteins (immunology), Virology.
- MESH :
- chemical , biosynthesis : Antibodies, Monoclonal, Antibodies, Viral.
- chemical , immunology : Antibodies, Monoclonal, Antibodies, Viral, Membrane Glycoproteins, Nucleocapsid Proteins, Viral Envelope Proteins, Viral Matrix Proteins, Viral Structural Proteins.
- immunology : SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Antibody Specificity, Cell Line, Chlorocebus aethiops, Epitope Mapping, Humans, Neutralization Tests, Spike Glycoprotein, Coronavirus, Vero Cells.
Abstract
Monoclonal antibodies (Mabs) against the Urbani strain of the SARS-associated coronavirus (SARS-CoV) were developed and characterized for reactivity to SARS-CoV and SARS-CoV S, N, M, and E proteins using enzyme-linked immunoabsorbent (ELISA), radioimmunoprecipitation, immunofluorescence, Western Blot and microneutralization assays. Twenty-six mAbs were reactive to SARS-CoV by ELISA, and nine were chosen for detailed characterization. Five mAbs reacted against the S protein, two against the M protein, and one each against the N and E proteins. Two of five S protein mAbs neutralized SARS-CoV infection of Vero E6 cells and reacted to an epitope within amino acids 490-510 in the S protein. While two of the three non-neutralizing antibodies recognized at second epitope within amino acids 270-350. The mAbs characterized should prove useful for developing SARS-CoV diagnostic assays and for studying the biology of infection and pathogenesis of disease.
Affiliations:
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Le document en format XML
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Ambrosino</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Massachusetts Biologic Laboratories, University of Massachusetts Medical School</s1><s2>Jamaica Plain, MA 02130</s2><s3>USA</s3><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Alvarez, Rene" sort="Alvarez, Rene" uniqKey="Alvarez R" first="Rene" last="Alvarez">Rene Alvarez</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>National Centers for Infectious Diseases, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Virus Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-09</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>18 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Callaway, Justin" sort="Callaway, Justin" uniqKey="Callaway J" first="Justin" last="Callaway">Justin Callaway</name><affiliation wicri:level="2"><inist:fA14 i1="03"><s1>Furman University</s1><s2>Greenville, SC 29613</s2><s3>USA</s3><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Sud</region></placeName></affiliation></author><author><name sortKey="Cavitt, Sheana" sort="Cavitt, Sheana" uniqKey="Cavitt S" first="Sheana" last="Cavitt">Sheana Cavitt</name><affiliation wicri:level="2"><inist:fA14 i1="03"><s1>Furman University</s1><s2>Greenville, SC 29613</s2><s3>USA</s3><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Sud</region></placeName></affiliation></author><author><name sortKey="Kamrud, Kurt" sort="Kamrud, Kurt" uniqKey="Kamrud K" first="Kurt" last="Kamrud">Kurt Kamrud</name><affiliation wicri:level="2"><inist:fA14 i1="04"><s1>AlphaVax Inc</s1><s2>Research Triangle Park, NC 27709</s2><s3>USA</s3><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Alterson, Harold" sort="Alterson, Harold" uniqKey="Alterson H" first="Harold" last="Alterson">Harold Alterson</name><affiliation wicri:level="2"><inist:fA14 i1="04"><s1>AlphaVax Inc</s1><s2>Research Triangle Park, NC 27709</s2><s3>USA</s3><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Smith, Jonathan" sort="Smith, Jonathan" uniqKey="Smith J" first="Jonathan" last="Smith">Jonathan Smith</name><affiliation wicri:level="2"><inist:fA14 i1="04"><s1>AlphaVax Inc</s1><s2>Research Triangle Park, NC 27709</s2><s3>USA</s3><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Harcoun, Jennifer L" sort="Harcoun, Jennifer L" uniqKey="Harcoun J" first="Jennifer L." last="Harcoun">Jennifer L. Harcoun</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>National Centers for Infectious Diseases, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Virus Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-09</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>18 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Congrong Miao" sort="Congrong Miao" uniqKey="Congrong Miao" last="Congrong Miao">CONGRONG MIAO</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>National Centers for Infectious Diseases, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Virus Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-09</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>18 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Razdan, Raj" sort="Razdan, Raj" uniqKey="Razdan R" first="Raj" last="Razdan">Raj Razdan</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>National Centers for Infectious Diseases, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Virus Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-09</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>18 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Comer, James A" sort="Comer, James A" uniqKey="Comer J" first="James A." last="Comer">James A. Comer</name><affiliation wicri:level="2"><inist:fA14 i1="05"><s1>National Center for Infectious Diseases, Division of Viral and Rickettsial Diseases, Special Pathogens Branch, Centers for Disease Control and Prevention</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>21 aut.</sZ><sZ>22 aut.</sZ><sZ>23 aut.</sZ><sZ>24 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Rollin, Pierre E" sort="Rollin, Pierre E" uniqKey="Rollin P" first="Pierre E." last="Rollin">Pierre E. Rollin</name><affiliation wicri:level="2"><inist:fA14 i1="05"><s1>National Center for Infectious Diseases, Division of Viral and Rickettsial Diseases, Special Pathogens Branch, Centers for Disease Control and Prevention</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>21 aut.</sZ><sZ>22 aut.</sZ><sZ>23 aut.</sZ><sZ>24 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Ksiazek, Thomas G" sort="Ksiazek, Thomas G" uniqKey="Ksiazek T" first="Thomas G." last="Ksiazek">Thomas G. Ksiazek</name><affiliation wicri:level="2"><inist:fA14 i1="05"><s1>National Center for Infectious Diseases, Division of Viral and Rickettsial Diseases, Special Pathogens Branch, Centers for Disease Control and Prevention</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>21 aut.</sZ><sZ>22 aut.</sZ><sZ>23 aut.</sZ><sZ>24 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Sanchez, Anthony" sort="Sanchez, Anthony" uniqKey="Sanchez A" first="Anthony" last="Sanchez">Anthony Sanchez</name><affiliation wicri:level="2"><inist:fA14 i1="05"><s1>National Center for Infectious Diseases, Division of Viral and Rickettsial Diseases, Special Pathogens Branch, Centers for Disease Control and Prevention</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>21 aut.</sZ><sZ>22 aut.</sZ><sZ>23 aut.</sZ><sZ>24 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Rota, Paul A" sort="Rota, Paul A" uniqKey="Rota P" first="Paul A." last="Rota">Paul A. Rota</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>National Centers for Infectious Diseases, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Virus Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-09</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>18 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Bellini, William J" sort="Bellini, William J" uniqKey="Bellini W" first="William J." last="Bellini">William J. Bellini</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>National Centers for Infectious Diseases, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Virus Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-09</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>18 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Anderson, Larry J" sort="Anderson, Larry J" uniqKey="Anderson L" first="Larry J." last="Anderson">Larry J. Anderson</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>National Centers for Infectious Diseases, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Virus Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-09</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>18 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">05-0355538</idno><date when="2005">2005</date><idno type="stanalyst">PASCAL 05-0355538 INIST</idno><idno type="RBID">Pascal:05-0355538</idno><idno type="wicri:Area/PascalFrancis/Corpus">000629</idno><idno type="wicri:Area/PascalFrancis/Curation">000361</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000606</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000606</idno><idno type="wicri:doubleKey">0166-0934:2005:Tripp R:monoclonal:antibodies:to</idno><idno type="wicri:Area/Main/Merge">005126</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:15885812</idno><idno type="wicri:Area/PubMed/Corpus">002750</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002750</idno><idno type="wicri:Area/PubMed/Curation">002750</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002750</idno><idno type="wicri:Area/PubMed/Checkpoint">002614</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002614</idno><idno type="wicri:Area/Ncbi/Merge">000F37</idno><idno type="wicri:Area/Ncbi/Curation">000F37</idno><idno type="wicri:Area/Ncbi/Checkpoint">000F37</idno><idno type="wicri:doubleKey">0166-0934:2005:Tripp R:monoclonal:antibodies:to</idno><idno type="wicri:Area/Main/Merge">004A23</idno><idno type="wicri:Area/Main/Curation">004D88</idno><idno type="wicri:Area/Main/Exploration">004D88</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV) : Identification of neutralizing and antibodies reactive to S, N, M and E viral proteins</title><author><name sortKey="Tripp, Ralph A" sort="Tripp, Ralph A" uniqKey="Tripp R" first="Ralph A." last="Tripp">Ralph A. Tripp</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>National Centers for Infectious Diseases, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Virus Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-09</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>18 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Haynes, Lia M" sort="Haynes, Lia M" uniqKey="Haynes L" first="Lia M." last="Haynes">Lia M. Haynes</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>National Centers for Infectious Diseases, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Virus Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-09</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>18 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Moore, Deborah" sort="Moore, Deborah" uniqKey="Moore D" first="Deborah" last="Moore">Deborah Moore</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>National Centers for Infectious Diseases, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Virus Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-09</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>18 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Anderson, Barbara" sort="Anderson, Barbara" uniqKey="Anderson B" first="Barbara" last="Anderson">Barbara Anderson</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>National Centers for Infectious Diseases, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Virus Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-09</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>18 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Tamin, Azaibi" sort="Tamin, Azaibi" uniqKey="Tamin A" first="Azaibi" last="Tamin">Azaibi Tamin</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>National Centers for Infectious Diseases, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Virus Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-09</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>18 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Harcourt, Brian H" sort="Harcourt, Brian H" uniqKey="Harcourt B" first="Brian H." last="Harcourt">Brian H. Harcourt</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>National Centers for Infectious Diseases, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Virus Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-09</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>18 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Jones, Les P" sort="Jones, Les P" uniqKey="Jones L" first="Les P." last="Jones">Les P. Jones</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>National Centers for Infectious Diseases, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Virus Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-09</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>18 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Yilla, Mamadi" sort="Yilla, Mamadi" uniqKey="Yilla M" first="Mamadi" last="Yilla">Mamadi Yilla</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>National Centers for Infectious Diseases, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Virus Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-09</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>18 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Babcock, Gregory J" sort="Babcock, Gregory J" uniqKey="Babcock G" first="Gregory J." last="Babcock">Gregory J. Babcock</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Massachusetts Biologic Laboratories, University of Massachusetts Medical School</s1><s2>Jamaica Plain, MA 02130</s2><s3>USA</s3><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Greenough, Thomas" sort="Greenough, Thomas" uniqKey="Greenough T" first="Thomas" last="Greenough">Thomas Greenough</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Massachusetts Biologic Laboratories, University of Massachusetts Medical School</s1><s2>Jamaica Plain, MA 02130</s2><s3>USA</s3><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Ambrosino, Donna M" sort="Ambrosino, Donna M" uniqKey="Ambrosino D" first="Donna M." last="Ambrosino">Donna M. Ambrosino</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Massachusetts Biologic Laboratories, University of Massachusetts Medical School</s1><s2>Jamaica Plain, MA 02130</s2><s3>USA</s3><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Alvarez, Rene" sort="Alvarez, Rene" uniqKey="Alvarez R" first="Rene" last="Alvarez">Rene Alvarez</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>National Centers for Infectious Diseases, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Virus Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-09</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>18 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Callaway, Justin" sort="Callaway, Justin" uniqKey="Callaway J" first="Justin" last="Callaway">Justin Callaway</name><affiliation wicri:level="2"><inist:fA14 i1="03"><s1>Furman University</s1><s2>Greenville, SC 29613</s2><s3>USA</s3><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Sud</region></placeName></affiliation></author><author><name sortKey="Cavitt, Sheana" sort="Cavitt, Sheana" uniqKey="Cavitt S" first="Sheana" last="Cavitt">Sheana Cavitt</name><affiliation wicri:level="2"><inist:fA14 i1="03"><s1>Furman University</s1><s2>Greenville, SC 29613</s2><s3>USA</s3><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Sud</region></placeName></affiliation></author><author><name sortKey="Kamrud, Kurt" sort="Kamrud, Kurt" uniqKey="Kamrud K" first="Kurt" last="Kamrud">Kurt Kamrud</name><affiliation wicri:level="2"><inist:fA14 i1="04"><s1>AlphaVax Inc</s1><s2>Research Triangle Park, NC 27709</s2><s3>USA</s3><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Alterson, Harold" sort="Alterson, Harold" uniqKey="Alterson H" first="Harold" last="Alterson">Harold Alterson</name><affiliation wicri:level="2"><inist:fA14 i1="04"><s1>AlphaVax Inc</s1><s2>Research Triangle Park, NC 27709</s2><s3>USA</s3><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Smith, Jonathan" sort="Smith, Jonathan" uniqKey="Smith J" first="Jonathan" last="Smith">Jonathan Smith</name><affiliation wicri:level="2"><inist:fA14 i1="04"><s1>AlphaVax Inc</s1><s2>Research Triangle Park, NC 27709</s2><s3>USA</s3><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Harcoun, Jennifer L" sort="Harcoun, Jennifer L" uniqKey="Harcoun J" first="Jennifer L." last="Harcoun">Jennifer L. Harcoun</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>National Centers for Infectious Diseases, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Virus Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-09</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>18 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Congrong Miao" sort="Congrong Miao" uniqKey="Congrong Miao" last="Congrong Miao">CONGRONG MIAO</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>National Centers for Infectious Diseases, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Virus Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-09</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>18 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Razdan, Raj" sort="Razdan, Raj" uniqKey="Razdan R" first="Raj" last="Razdan">Raj Razdan</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>National Centers for Infectious Diseases, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Virus Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-09</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>18 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Comer, James A" sort="Comer, James A" uniqKey="Comer J" first="James A." last="Comer">James A. Comer</name><affiliation wicri:level="2"><inist:fA14 i1="05"><s1>National Center for Infectious Diseases, Division of Viral and Rickettsial Diseases, Special Pathogens Branch, Centers for Disease Control and Prevention</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>21 aut.</sZ><sZ>22 aut.</sZ><sZ>23 aut.</sZ><sZ>24 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Rollin, Pierre E" sort="Rollin, Pierre E" uniqKey="Rollin P" first="Pierre E." last="Rollin">Pierre E. Rollin</name><affiliation wicri:level="2"><inist:fA14 i1="05"><s1>National Center for Infectious Diseases, Division of Viral and Rickettsial Diseases, Special Pathogens Branch, Centers for Disease Control and Prevention</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>21 aut.</sZ><sZ>22 aut.</sZ><sZ>23 aut.</sZ><sZ>24 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Ksiazek, Thomas G" sort="Ksiazek, Thomas G" uniqKey="Ksiazek T" first="Thomas G." last="Ksiazek">Thomas G. Ksiazek</name><affiliation wicri:level="2"><inist:fA14 i1="05"><s1>National Center for Infectious Diseases, Division of Viral and Rickettsial Diseases, Special Pathogens Branch, Centers for Disease Control and Prevention</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>21 aut.</sZ><sZ>22 aut.</sZ><sZ>23 aut.</sZ><sZ>24 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Sanchez, Anthony" sort="Sanchez, Anthony" uniqKey="Sanchez A" first="Anthony" last="Sanchez">Anthony Sanchez</name><affiliation wicri:level="2"><inist:fA14 i1="05"><s1>National Center for Infectious Diseases, Division of Viral and Rickettsial Diseases, Special Pathogens Branch, Centers for Disease Control and Prevention</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>21 aut.</sZ><sZ>22 aut.</sZ><sZ>23 aut.</sZ><sZ>24 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Rota, Paul A" sort="Rota, Paul A" uniqKey="Rota P" first="Paul A." last="Rota">Paul A. Rota</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>National Centers for Infectious Diseases, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Virus Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-09</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>18 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Bellini, William J" sort="Bellini, William J" uniqKey="Bellini W" first="William J." last="Bellini">William J. Bellini</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>National Centers for Infectious Diseases, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Virus Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-09</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>18 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Anderson, Larry J" sort="Anderson, Larry J" uniqKey="Anderson L" first="Larry J." last="Anderson">Larry J. Anderson</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>National Centers for Infectious Diseases, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Virus Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-09</s1><s2>Atlanta, GA 30333</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ><sZ>18 aut.</sZ><sZ>19 aut.</sZ><sZ>20 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Journal of virological methods</title><title level="j" type="abbreviated">J. virol. methods</title><idno type="ISSN">0166-0934</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virological methods</title><title level="j" type="abbreviated">J. virol. methods</title><idno type="ISSN">0166-0934</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal (biosynthesis)</term><term>Antibodies, Monoclonal (immunology)</term><term>Antibodies, Viral (biosynthesis)</term><term>Antibodies, Viral (immunology)</term><term>Antibody Specificity</term><term>Antigenic determinant</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Epitope Mapping</term><term>Humans</term><term>Identification</term><term>Membrane Glycoproteins (immunology)</term><term>Method</term><term>Microbiology</term><term>Monoclonal antibody</term><term>Neutralization Tests</term><term>Neutralizing antibody</term><term>Nucleocapsid Proteins (immunology)</term><term>Protein</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Severe acute respiratory syndrome virus</term><term>Spike Glycoprotein, Coronavirus</term><term>Vero Cells</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Matrix Proteins (immunology)</term><term>Viral Structural Proteins (immunology)</term><term>Virology</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (biosynthèse)</term><term>Anticorps antiviraux (immunologie)</term><term>Anticorps monoclonaux (biosynthèse)</term><term>Anticorps monoclonaux (immunologie)</term><term>Cartographie épitopique</term><term>Cellules Vero</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (immunologie)</term><term>Humains</term><term>Lignée cellulaire</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Protéines de la matrice virale (immunologie)</term><term>Protéines nucléocapside (immunologie)</term><term>Protéines virales structurales (immunologie)</term><term>Spécificité des anticorps</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Tests de neutralisation</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antibodies, Viral</term><term>Membrane Glycoproteins</term><term>Nucleocapsid Proteins</term><term>Viral Envelope Proteins</term><term>Viral Matrix Proteins</term><term>Viral Structural Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps monoclonaux</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps monoclonaux</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines de la matrice virale</term><term>Protéines nucléocapside</term><term>Protéines virales structurales</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Antibody Specificity</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Epitope Mapping</term><term>Humans</term><term>Neutralization Tests</term><term>Spike Glycoprotein, Coronavirus</term><term>Vero Cells</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term><term>Cartographie épitopique</term><term>Cellules Vero</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Lignée cellulaire</term><term>Spécificité des anticorps</term><term>Tests de neutralisation</term><term>Virus syndrome respiratoire aigu sévère</term><term>Anticorps monoclonal</term><term>Identification</term><term>Anticorps neutralisant</term><term>Protéine</term><term>Déterminant antigénique</term><term>Microbiologie</term><term>Méthode</term><term>Virologie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Monoclonal antibodies (Mabs) against the Urbani strain of the SARS-associated coronavirus (SARS-CoV) were developed and characterized for reactivity to SARS-CoV and SARS-CoV S, N, M, and E proteins using enzyme-linked immunoabsorbent (ELISA), radioimmunoprecipitation, immunofluorescence, Western Blot and microneutralization assays. Twenty-six mAbs were reactive to SARS-CoV by ELISA, and nine were chosen for detailed characterization. Five mAbs reacted against the S protein, two against the M protein, and one each against the N and E proteins. Two of five S protein mAbs neutralized SARS-CoV infection of Vero E6 cells and reacted to an epitope within amino acids 490-510 in the S protein. While two of the three non-neutralizing antibodies recognized at second epitope within amino acids 270-350. The mAbs characterized should prove useful for developing SARS-CoV diagnostic assays and for studying the biology of infection and pathogenesis of disease.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Caroline du Nord</li><li>Caroline du Sud</li><li>Géorgie (États-Unis)</li><li>Massachusetts</li></region></list><tree><country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Tripp, Ralph A" sort="Tripp, Ralph A" uniqKey="Tripp R" first="Ralph A." last="Tripp">Ralph A. Tripp</name></region><name sortKey="Alterson, Harold" sort="Alterson, Harold" uniqKey="Alterson H" first="Harold" last="Alterson">Harold Alterson</name><name sortKey="Alvarez, Rene" sort="Alvarez, Rene" uniqKey="Alvarez R" first="Rene" last="Alvarez">Rene Alvarez</name><name sortKey="Ambrosino, Donna M" sort="Ambrosino, Donna M" uniqKey="Ambrosino D" first="Donna M." last="Ambrosino">Donna M. Ambrosino</name><name sortKey="Anderson, Barbara" sort="Anderson, Barbara" uniqKey="Anderson B" first="Barbara" last="Anderson">Barbara Anderson</name><name sortKey="Anderson, Larry J" sort="Anderson, Larry J" uniqKey="Anderson L" first="Larry J." last="Anderson">Larry J. Anderson</name><name sortKey="Babcock, Gregory J" sort="Babcock, Gregory J" uniqKey="Babcock G" first="Gregory J." last="Babcock">Gregory J. Babcock</name><name sortKey="Bellini, William J" sort="Bellini, William J" uniqKey="Bellini W" first="William J." last="Bellini">William J. Bellini</name><name sortKey="Callaway, Justin" sort="Callaway, Justin" uniqKey="Callaway J" first="Justin" last="Callaway">Justin Callaway</name><name sortKey="Cavitt, Sheana" sort="Cavitt, Sheana" uniqKey="Cavitt S" first="Sheana" last="Cavitt">Sheana Cavitt</name><name sortKey="Comer, James A" sort="Comer, James A" uniqKey="Comer J" first="James A." last="Comer">James A. Comer</name><name sortKey="Congrong Miao" sort="Congrong Miao" uniqKey="Congrong Miao" last="Congrong Miao">CONGRONG MIAO</name><name sortKey="Greenough, Thomas" sort="Greenough, Thomas" uniqKey="Greenough T" first="Thomas" last="Greenough">Thomas Greenough</name><name sortKey="Harcoun, Jennifer L" sort="Harcoun, Jennifer L" uniqKey="Harcoun J" first="Jennifer L." last="Harcoun">Jennifer L. Harcoun</name><name sortKey="Harcourt, Brian H" sort="Harcourt, Brian H" uniqKey="Harcourt B" first="Brian H." last="Harcourt">Brian H. Harcourt</name><name sortKey="Haynes, Lia M" sort="Haynes, Lia M" uniqKey="Haynes L" first="Lia M." last="Haynes">Lia M. Haynes</name><name sortKey="Jones, Les P" sort="Jones, Les P" uniqKey="Jones L" first="Les P." last="Jones">Les P. Jones</name><name sortKey="Kamrud, Kurt" sort="Kamrud, Kurt" uniqKey="Kamrud K" first="Kurt" last="Kamrud">Kurt Kamrud</name><name sortKey="Ksiazek, Thomas G" sort="Ksiazek, Thomas G" uniqKey="Ksiazek T" first="Thomas G." last="Ksiazek">Thomas G. Ksiazek</name><name sortKey="Moore, Deborah" sort="Moore, Deborah" uniqKey="Moore D" first="Deborah" last="Moore">Deborah Moore</name><name sortKey="Razdan, Raj" sort="Razdan, Raj" uniqKey="Razdan R" first="Raj" last="Razdan">Raj Razdan</name><name sortKey="Rollin, Pierre E" sort="Rollin, Pierre E" uniqKey="Rollin P" first="Pierre E." last="Rollin">Pierre E. Rollin</name><name sortKey="Rota, Paul A" sort="Rota, Paul A" uniqKey="Rota P" first="Paul A." last="Rota">Paul A. Rota</name><name sortKey="Sanchez, Anthony" sort="Sanchez, Anthony" uniqKey="Sanchez A" first="Anthony" last="Sanchez">Anthony Sanchez</name><name sortKey="Smith, Jonathan" sort="Smith, Jonathan" uniqKey="Smith J" first="Jonathan" last="Smith">Jonathan Smith</name><name sortKey="Tamin, Azaibi" sort="Tamin, Azaibi" uniqKey="Tamin A" first="Azaibi" last="Tamin">Azaibi Tamin</name><name sortKey="Yilla, Mamadi" sort="Yilla, Mamadi" uniqKey="Yilla M" first="Mamadi" last="Yilla">Mamadi Yilla</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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